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Cas9 Virus Particle

For permanent expression, sgRNA and Cas9 can be cloned into a lentiviral vector, packaged into viral particles, and transduced into target cells. Since both sgRNA and Cas9 are stably integrated into the host cell genome, they are passed on to the progeny cells. In 2014, studies (Wang et al., Science; Shalem et al., Science) showed that a library of CRISPR/Cas9 lentiviral constructs can be used to identify genes essential for mammalian cells. Moreover, CRISPR/Cas9 virus particles also can be used to help scientists create stable knockout/knock-in cell lines.

Posted on : Fri, Dec 13, 2024 8:53 AM

Posted By : Marcia Brady